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Image Search Results
Journal: Cell reports
Article Title: Flattening of circadian glucocorticoid oscillations drives acute hyperinsulinemia and adipocyte hypertrophy
doi: 10.1016/j.celrep.2022.111018
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Thermogenin (UCP1) α-Diagnostics Cat#UCP11-A; RRID:AB_1876090 Mouse monoclonal β-Actin (HRP conjugated) Santa Cruz Cat#sc-47778 HRP; RRID:AB_2714189 Goat anti-rabbit IgG Cell Signaling Cat#7074; RRID:AB_2099233 Guinea pig anti-insulin IgG Dako Cat#IR002; RRID:AB_2800361 Rabbit anti-Ki-67 IgG Invitrogen Cat#MA5-14520; RRID:AB_10979488 Goat anti-guinea pig IgG Jackson ImmunoResearch Cat#106-545-003; RRID:AB_2337438 Goat anti-rabbit IgG Invitrogen Cat#A11036; RRID:AB_10563566 Chemicals, peptides, and recombinant proteins Corticosterone pellets (5mg, 21-day release) Innovative Research of America Cat#G-111 Placebo pellets (5mg, 21-day release) Innovative Research of America Cat#C-111 Corticosterone complexed with 2-hydroxypropyl-β-cyclodextrin Sigma-Aldrich Cat#C174 Glucose Sigma-Aldrich Cat#G8270 Insulin Sigma-Aldrich Cat#I9278 Normal Goat Serum Jackson ImmnuoResearch Cat#005-000-121 SlowFade™ Gold Antifade Mountant with DAPI Thermo Scientific Cat#S36939 Critical commercial
Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Quantitation Assay, SYBR Green Assay, Software
Journal: Cell reports
Article Title: Flattening of circadian glucocorticoid oscillations drives acute hyperinsulinemia and adipocyte hypertrophy
doi: 10.1016/j.celrep.2022.111018
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Thermogenin (UCP1) α-Diagnostics Cat#UCP11-A; RRID:AB_1876090 Mouse monoclonal β-Actin (HRP conjugated) Santa Cruz Cat#sc-47778 HRP; RRID:AB_2714189 Goat anti-rabbit IgG Cell Signaling Cat#7074; RRID:AB_2099233 Guinea pig anti-insulin IgG Dako Cat#IR002; RRID:AB_2800361 Rabbit anti-Ki-67 IgG Invitrogen Cat#MA5-14520; RRID:AB_10979488 Goat anti-guinea pig IgG Jackson ImmunoResearch Cat#106-545-003; RRID:AB_2337438 Goat anti-rabbit IgG Invitrogen Cat#A11036; RRID:AB_10563566 Chemicals, peptides, and recombinant proteins Corticosterone pellets (5mg, 21-day release) Innovative Research of America Cat#G-111 Placebo pellets (5mg, 21-day release) Innovative Research of America Cat#C-111 Corticosterone complexed with 2-hydroxypropyl-β-cyclodextrin Sigma-Aldrich Cat#C174 Glucose Sigma-Aldrich Cat#G8270 Insulin Sigma-Aldrich Cat#I9278 Normal Goat Serum Jackson ImmnuoResearch Cat#005-000-121 SlowFade™ Gold Antifade Mountant with DAPI Thermo Scientific Cat#S36939 Critical commercial
Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Quantitation Assay, SYBR Green Assay, Software
Journal: bioRxiv
Article Title: A novel mouse model of postpartum depression and the neurobiological effects of fast-acting antidepressant treatments
doi: 10.1101/2022.05.06.490916
Figure Lengend Snippet: (A) Schematic representation of the experimental timeline. Grey (SN) and pink (MSEW) lines represent (B) the percentage of females that retrieved the first pup during the test. (C) Proportion of females that retrieved (filled bar) all pups. (D) Number of pups that were retrieved. Representative photographs of nests built by SN (F) and MSEW (G) mothers. (E) Dispersion of the pups by their respective dams is shown as the occupied area of the nest (H) and the average distance of the pups from the centroid from each litter (X mean ; I) of SN and MSEW groups. (J) Time spent digging the sawdust. (K) Immobility time spent in TST and (L) self-grooming behaviour on splash test. (M) Content of allopregnanolone in plasma samples of SN and MSEW dams. pTRKB and BDNF protein levels in PFC (N,O) and hippocampus (P,Q). All data are represented as the mean ± SEM. (Log-rank test * p < 0.05; Fischer’s test *** p < 0.001; t-Student’s test, * p < 0.05, *** p < 0.001, Mann-Whitney’s test *** p < 0.001). MS, maternal separation; MSEW, maternal separation with early weaning; PD, postpartum day; PFC, prefrontal cortex; PRT, pup retrieval test; PW, postweaning day; SN, standard nest; SpT, splash test; TST, tail suspension test; W, day of weaning day.
Article Snippet: Samples selected for ELISA were processed according to the protocol supplied by the ELISA kit manufacturer for
Techniques:
Journal: bioRxiv
Article Title: A novel mouse model of postpartum depression and the neurobiological effects of fast-acting antidepressant treatments
doi: 10.1101/2022.05.06.490916
Figure Lengend Snippet: (A) Timeline of the experiments. Grey (SN) and pink (MSEW) dots represent (B) the time that animals are immobile in TST after vehicle, ketamine or allopregnanolone treatments (n = 10-12 / group). (C) Graph shows the cumulative time self-grooming behaviour of SN (grey dots) and MSEW (pink dots) dams in splash test after vehicle, ketamine or allopregnanolone treatments (n = 10-12 / group). All data are represented as the mean ± SEM. (Two-way ANOVA * p < 0.05, ** p < 0.01, *** p < 0.001). Allop, allopregnanolone; BrdU, 5-bromo-2’Deoxyuridine; Ket, ketamine; MS, maternal separation; MSEW, maternal separation with early weaning; perf, perfusion; PD, post-partum day; PRT, pup retrieval test; PW, post-weaning day; SN, standard nest; SPT, splash test; TST, tail suspension test; W, day of weaning day.
Article Snippet: Samples selected for ELISA were processed according to the protocol supplied by the ELISA kit manufacturer for
Techniques:
Journal: bioRxiv
Article Title: A novel mouse model of postpartum depression and the neurobiological effects of fast-acting antidepressant treatments
doi: 10.1101/2022.05.06.490916
Figure Lengend Snippet: (A) Timeline of the experiments. Grey (SN) and pink (MSEW) dots represent (B) the average of BrdU-positive cells per hemisphere after vehicle, ketamine or allopregnanolone treatments (n = 5-6 / group). Representative of single stack of BrdU (green cells) and NeuN-BrdU merge of vehicle- (images of SN female are C and C’; images of MSEW female are D and D’), ketamine- (images of SN female are E and E’; images of MSEW group are F and F’) and allopregnanolone-treated animals (images of SN female are G and G’; images of MSEW female are H and H’). All data are represented as the mean ± SEM. (Two-way ANOVA * p < 0.05, *** p < 0.001). Scale bar 100 μm. Allop, allopregnanolone; BrdU, 5-bromo-2’Deoxyuridine; Ket, ketamine; MS, maternal separation; MSEW, maternal separation with early weaning; Perf, perfusion; PD, postpartum day; PW, postweaning day; SN, standard nest; SpT, splash test; TST, tail suspension test; W, day of weaning day.
Article Snippet: Samples selected for ELISA were processed according to the protocol supplied by the ELISA kit manufacturer for
Techniques:
Journal: bioRxiv
Article Title: A novel mouse model of postpartum depression and the neurobiological effects of fast-acting antidepressant treatments
doi: 10.1101/2022.05.06.490916
Figure Lengend Snippet: (A) Timeline of the experiments. Grey (SN) and white (MSEW) bars represent the average number of (B) VGLUT1-, (C) VGAT-positive puncta in IL and (D) excitatory/inhibitory ratio (n = 5-6 / group) after vehicle, ketamine or allopregnanolone treatments measured in two different confocal z-planes in both hemispheres of each individual animal. Representative single confocal planes of puncta expressing excitatory (green, VGLUT1, E-J) and inhibitory (red, VGAT, E’-J’) synaptic markers in IL. All data are represented as the mean ± SEM. (Two-way ANOVA * p < 0.05, *** p < 0.001). Scale bar 50 μm. Allop, allopregnanolone; BrdU, 5-bromo-2’Deoxyuridine; Ket, ketamine; MS, maternal separation; MSEW, maternal separation with early weaning; Perf, perfusion; PD, postpartum day; PW, postweaning day; SN, standard nest; SpT, splash test; TST, tail suspension test; VGAT, vesicular GABA transporter; VGLUT1, vesicular glutamate transporter type 1W, day of weaning day.
Article Snippet: Samples selected for ELISA were processed according to the protocol supplied by the ELISA kit manufacturer for
Techniques: Expressing